The German giants recorded a 3-2 victory over the Europa League holders in the first leg last month and are therefore the favourites to advance to the final eight of the competition.
Dortmund fell behind to Sevilla in the first leg at Estadio Ramon, but Mahmoud Dahoud levelled the scores in the 19th minute before Erling Braut Haaland netted twice before the interval to make it 3-1.
Sevilla scored a second late on through Luuk de Jong to hand themselves a chance ahead of the second leg, but BVB will fancy their chances of finishing the job inside their own stadium to make the quarter-finals.
Dortmund picked up 13 points from their six Group F matches to top their section, and they have actually won nine of their last 14 European matches during an impressive run of form.
Last season, the Bundesliga giants reached the round of 16, losing to eventual finalists Paris Saint-Germain, and they have not progressed to the quarter-finals since the 2016-17 campaign, where they were beaten by Ligue 1 side Monaco.
Edin Terzic’s side will enter Tuesday’s clash off the back of a 4-2 defeat to Bayern Munich in the Bundesliga, which has left them down in sixth position in the table, four points off the top four.
Sevilla are far from out of the last-16 tie, but they not enter this contest in the best of form, having lost four of their last five matches in all competitions, including a shock 2-1 loss at Elche in La Liga on Saturday.
Julen Lopetegui’s side are now only six points clear of fifth-placed Real Sociedad in Spain’s top flight, while they lost 3-0 to Barcelona in the second leg of their Copa del Rey semi-final on March 3 to suffer a 3-2 aggregate defeat.
Sevilla are only actually in their sixth Champions League campaign, but they made the quarter-finals in 2017-18, while the Spanish outfit are the most successful team in the history of the UEFA Cup/Europa League, winning the European competition on six occasions, including last term.
Lopetegui’s team impressed in the group stages this season, picking up 13 points to finish second behind Chelsea in Group E, while they have lost just six of their 24 games against German opposition in European competition, which will hand them confidence heading into this game.
Los Nervionenses also recorded a 1-0 victory when they last travelled to Dortmund during the group stages of the 2010-11 Europa League, although the same scoreline on Tuesday would see them lose on away goals.
Borussia Dortmund Champions League form:WWWDWW
Borussia Dortmund form (all competitions): DWWWWL
Sevilla Champions League form: WWWLWL
Sevilla form (all competitions): WLWLLL